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The deflection of the cell with gel electrolyte was smaller than that of the cell with aqueous solution electrolyte.
Binding of T4P to the pilus-coated surface and subsequent retraction led to a deflection of the cell body.
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This paper presents calibration results from three investigated methods as well as results from a finite element analysis of the plate deflection of the pressure cell which were performed to evaluate the non-linear behaviour of the signal from the cell.
Figure 4c and 4e(iv) show large deviations in the deflection of the cantilever when cells are indented for longer time periods.
Nevertheless, i have a technical concern and some questions that i feel the authors could have discussed more in-depth in the manuscript: Figure 4c and 4e(iv) show large deviations in the deflection of the cantilever when cells are indented for longer time periods.
Sound, movement and acceleration cause deflection of hair cell apical projections, named stereocilia, located at the luminal surface of the epithelium.
It was therefore important to have plates in close proximity to the deflection point of the single cell and a rigid sort plate setup that does not move during the sort.
Images show 3D reconstructions of AFM height images (not shown) with the corresponding deflection images and height profiles of the cell body along the indicated red lines.
This was confirmed by FE simulations of the interaction between the stereocilia and the tectorial membrane, when this anisotropy was incorporated into the model, thus providing an additional mechanism for the deflection of outer hair cell stereocilia apart from viscous fluid forces.
A cantilever probe is used to tap gently on the surface of the cell; the deflection of the cantilever is proportional to the stiffness of the region being tapped.
(A and B) Bioforce probe (BFP, A) [ 31,71] or atomic force microscopy (AFM, B) [ 2,7 ] have been used to determine de-adhesion forces of molecules and cells by measuring the deflection of the cantilever (AFM) or the deformation of a pressurized red blood cell (BFP).
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