To definitively show that the luciferase/GFP expressing cells were functional stem cells, single visualized GFPhi cells were transplanted into cleared mammary fat pads.
Here we show more definitively that Foxc1 is expressed by brain pericytes and that it is essential for normal pericyte function in the developing brain.
To definitively determine whether physiologically expressed FLT3-ITD impacts the establishment of myeloid versus lymphoid leukemia development, we crossed Flt3 ITD/ITD with Runx1fl/fl (Growney et al., 2005) and Mx1-Cre mice to induce Runx1 deletion in MPPs.
However, because our previous study did not have data from pupal stages examining gene expression in each of the sexes separately, or in male and female tud progeny, we were unable to definitively identify the genes expressed in the female germline at pupal stages.
These data definitively show that the developing male reproductive tract, although perhaps not dependent on estrogens or ER for normal development, expresses ER and is quite sensitive to the toxicological effects of exogenous estrogens when aberrantly exposed.
These results, coupled with those of the cis-regulatory analysis reported previously (Cavalieri et al., 2008), lead us to definitively conclude that hbox12 is expressed in the prospective dorsal ectoderm of the early embryo.
In order to characterize definitively the range of FGFR2 variants expressed by SUM-52PE cells, individual isoforms were cloned and sequenced from SUM-52PE RNA.
Because the endogenous protein is not mosaically expressed, it is not possible to definitively resolve the expression to either the anterior or posterior face of the cells.
In order to definitively ascertain the functional contribution of lymphotoxin (LT) expressed by B cells, we produced mice with the LTβ gene deleted from B cells (B-LTβ KO mice).
These results establish definitively that BNP and NPR-A are specifically expressed in self-renewing ES cells.
