To definitively prove this conclusion, we applied CRISPR/Cas9 genome engineering to knock out APN expression in PEDV-susceptible porcine (ST) and human cell lines (Huh7 and HeLa).
In order to definitively explore this issue, we applied the Francesconi (2014) method to explicitly identify genotype x time interactions.
Thus, whether PVG and IP are caused by infection or mechanical mucosal rupture should be definitively determined, and treatment should be applied based on pathological conditions.
These observations are consistent with this region folding as a globular domain, but biophysical and structural approaches must now be applied to determine this definitively.
Identification of Pfiesteria-like organisms is a particularly difficult element of the monitoring programs, as these small species cannot be definitively identified using light microscopy; newly applied molecular techniques, however, are starting to provide alternatives to traditional methods.
His spiritual crisis reached its climax and the point of no return in 1872, when he definitively abandoned the project of becoming a minister and applied as a non-collegiate student at Cambridge.
Because only one of the molecular criteria (overall fingerprint pattern) could be applied, isolates with these patterns cannot be definitively called W-Beijing.
As noted above, given the fact that the parents who participated in ECSEL were primarily middle/upper middle class, the degree to which our program can be applied to families of lower income levels cannot be definitively determined.
The term, much debated and sometimes applied to other plays, remains in use, though Hamlet is definitively classed as a tragedy.
First applied in Escherichia coli [ 4] and Hela cells [ 5], the visualization of protein interactions in vivo was definitively validated by the work of Hu et al., who investigated the interactions between transcription factors in mammalian cells [ 6].
