However, more detailed studies combining quantitative RT-PCR and Western blot using multiple cell lines and a large number of incubation times will be necessary to clearly define the expression of TRF2 after DNA damage.
Since the expression HIF-1α, VEGF and EPO in newborns diagnosed with these diseases has yet to be studied, we set out to define the expression of these genes in peripheral blood from newborn infants diagnosed with PPHN and CCHD.
Methods: Northern analysis, in situ hybridization, RT-PCR analysis, and immunoprecipitation were used to define the expression of IL-13Rα1 and IL-13Rα2 in lungs from lung targeted overexpression mice and lung fragments and cells in culture.
Here, we generated two monoclonal antibodies (MAbs), 5E3 and 14G8, that are specific for human LSP1 to define the expression of LSP1 throughout human T-cell development.
The classical concept asked the question regarding the feasibility of finely optimizing the spatio-temporal expression of a regulon that defines the expression of a complex phenotype such as drought tolerance by driving its major regulatory gene (i.e., regulatory hub) under the control of a novel and ideal chimeric promoter.
To define the expression of MS4a4B, we detected MS4a4B protein in mouse cells, including normal T cells, non-T cells, and malignant T cells, by using anti-MS4a4B antibodies and flow cytometric analysis.
Both the intensity and percentage of positivity were assessed to define the expression of MMP13.
The Affymetrix platform utilizes multiple probes assembled into probe sets to define the expression of genes.
In the gated cells we further defined the expression of CD11c and CD123 to determine the two distinct DC lineages.
Quantitative real-time PCR (qRT-PCR) was utilized to define the expression of cylinD1, axinII, and myc.
