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The phrase "defining difference" is a perfectly acceptable phrase in written English.
You can use it to refer to a specific characteristic that creates a distinction between two things or people. For example, you could say, "The defining difference between these two pieces of software lies in their user interface design."
Exact(40)
Building great startups begins with defining difference with the companies already in a marketplace, but starting out different is not enough.
That's an important, if not the defining, difference.
The defining difference, quite simply, was that Augusta County had slavery, and Franklin County did not.
However, I'm not sure that's the defining difference between then and now.
He sees these acts of memory as a defining difference within Europe.
Here is a defining difference between practice under Mayor Johnson and aspiration under Mayor Khan.
Similar(20)
Whatever similarities the series implies, Mr. Hughes is most emphatic about the great defining differences between America and Australia.
Each member of the ensemble here embodies this shared storytelling spirit with vivid defining differences, without overselling the surface eccentricities.
Facebook (almost counterintuitively) is the one that favors an algorithmic approach, and currently it's one of the defining differences between Facebook and G+.
Dual crossovers between ERS-2 and TOPEX/Poseidon provide a measure of the relative bias along with other parameters defining differences between the altimetric data sets.
As the Scorecard reveals, there are still defining differences among the leading Democratic contenders.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com