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In the report, the number of cycles required to reach threshold fluorescence (Ct) and ΔCT for each sample relative to the control gene defines the expression pattern for a gene.
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To define the expression pattern of PsAvr3c during P. sojae infection, we performed quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) at 0.5,1,2,3,6,24 and 36 hours post inoculation (hpi) on susceptible soybean.
To precisely define the expression pattern of OsAP77 after blast fungus infection, we examined the pattern of OsAP77::GUS expression in 6-week-old seedlings.
These lines defined the expression pattern and onset of reporter gene expression.
We compared these data with our miRNA expression data to define the expression pattern of the stomach tissue.
Quantitative immunofluorescence further defined the expression pattern of BK channels relative to SK channels and efferent innervation.
We sought to define the expression pattern of FGF20 to confirm its role in feather development.
Semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) studies were undertaken to define the expression pattern for D. virilis RNAs generated in the y ac intergenic region.
To define the expression pattern of these genes in the various sugarcane tissues cDNA microarrays with 1,280 distinct elements were constructed.
To drive a transgene of interest in the desired tissue, one has to decide which promoter to choose as this will define the expression pattern.
To define the expression pattern of Perp within these layers, we co-stained mammary gland sections for Perp and SMA to highlight myoepithelial cells [ 29], and for Perp together with keratin 8 to mark luminal epithelial cells [ 30].
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CEO of Professional Science Editing for Scientists @ prosciediting.com