A 3D mechanical digitizer (MicroScribe G2X, Immersion, San Jose, CA) was used to collect a cloud of about 2 thousand points on the outer surface of the specimen, which were used to define specimen geometry.
In analyses of long-term specimens (defined as specimens from seroconverters infected in prior years), data were adjusted for misclassification by using a recently described formula [17].
The IR group was defined as specimens belonging to neither the HR nor the LR groups.
Surveillance samples are defined as specimens obtained from body sites were PPM are normally carried by the digestive tract.
We defined 49 specimens with scores 2 and 3 as positive expression and 43 with scores 0 and 1 as negative with FoxP3.
Extent and intensity scores were multiplied to give total immunohistochemical (IHC) scores, ranging from 0 to 8. GPR30+ expression was defined for specimens that scored ≥2.
For these countries, we defined postpandemic specimens as those collected at least 2 weeks after the date on which 90% of 2009 laboratory notifications had occurred for the region.
We defined 11 specimens as acute SARS (AS) using the following criteria: (i) the whole blood RNA from a hospitalized patient was PCR positive for SARS-CoV, or (ii) the specimen was collected within 10 days after the disease onset in patients whose blood was later diagnosed ELISA-positive for anti-SARS IgG.
In contrast, microvilli were well defined in specimens obtained from those animals treated with Plantago ovata husk and acetylsalicylic acid: they were similar to those observed in the control group as well, without apparent signs of damage (Fig. 2(c) and (f)).
In a hypothesis-driven approach, we determined the expression levels of TACE and TIMP3 mRNA by in situ hybridisation in a training set comprising a series of commercially supplied histopathologically defined HNSCC specimens (HNSCC tissue micro array; US Biomax, Inc., Rockville, MD, USA).
For the analysis of each parameter in the infill wall OOP performance, three different groups were defined: as built specimens, specimens with previous in-plane damage and retrofitted specimens.
