Sentence examples for defined nucleotide from inspiring English sources

RNA virus genomes do not replicate as a defined nucleotide sequence, but as complex and dynamic mutant spectra termed viral quasispecies (reviews in [1], [2]).

Recent bioinformatics studies have revealed that most mammalian genes do not conform to the simple model in which a TATA box directs transcription from a single defined nucleotide position –most genes have multiple promoters, within which there are multiple start sites, and that 72% of human promoters are associated with CpG islands [1] [3].

However, instead of randomized ssDNA molecules, a fixed sequence with a defined nucleotide sequence containing primer hybridization regions could also be used.

Assuming that any nucleotide substitution at a defined nucleotide site shifts locus S from the advantageous to the disadvantageous state, we defined μ = 2.5 × 10-5 per generation.

Alignment of our sequencing results with 62 different T. gondii isolates (5 reference strains, 7 clinical isolates and 51 T. gondii-positive amniotic fluids) revealed 3 nucleotide mutations, one deletion, and one defined nucleotide ambiguity compared to GenBank AF146527.

Accordingly, we defined a cut off for the description of true heterogeneity at a defined nucleotide position as ≥30%% i.e. if a variant identified in at least 30%% of the Illumina sequencing reads (after filtering and GenomeView analysis), it is likely that the variant is truly present within the genome of a sub-population of bacilli.

Raw data is preprocessed before de novo assembly: low-quality nucleotides (we defined nucleotides with a quality score less than 20 as low-quality nucleotides) in the last 20 cycles and ambiguous nucleotides in the first five cycles were trimmed by custom PERL script.

For the detection of the retinoic acid-response element (RARE binding sites, we did an exact forward and reverse search of 18 experimentally defined DR5 RAREs (Mainguy et al. 2003) that consists of five defined nucleotides on each side separated by five arbitrary nucleotides.

The constructs define nucleotide transition rates and they are applied to choose starting sequences to yield specific nonrandom pools.

To produce the targeting construct, we PCR amplified two trpm genomic regions from w1118 adult flies (5' end of C1 exon defined as nucleotide 1: left arm, 4937 kb, nucleotides −3600 to 1337; right arm, 5022 kb, nucleotides 2980 to 8001), which we subcloned into a modified version of the p[w25] targeting plasmid [18].

Clonal groups should be defined using nucleotide sequences, and not amino acids, since the rearrangement process and SHM operate at the nucleotide level.