Once it has defined minimal coverage, the government should step aside.
We test that the difference is bigger than an a priori defined minimal meaningful effect (MME).
Here, S. oneidensis MR-1 was cultured in a chemically defined minimal medium as described previously [11].
These values were obtained in a defined minimal medium with a prototrophic and plasmid-free strain, making this process highly interesting for industrial application.
700 mL of defined minimal medium were inoculated with an amount of pre-culture that resulted in a final OD600 of 0.02.
Shewanella oneidensis MR-1 and BFO membranes were cultured and synthesized in a chemically defined minimal medium as described previously [12].
For most dry powder inhalers (DPIs) a defined minimal inspiratory flow must be achieved immediately after the start of the inspiration procedure, to avoid insufficient deposition in the lung and excessive drug deposition in the patient's mouth and/or oropharynx.
More specifically, liquid media were chemically defined minimal medium, general medium and UHT skimmed milk; gelatine and a gelatine-dextran mixture were the gelling agents used, leading respectively a homogeneous and heterogeneous solid system.
They found that four strains were grown and sporulated in a simple chemically defined minimal medium, however, the remaining strains needed vitamins or amino acids as well as purine.
After 15 h, the culture was transferred into 50 mL of defined minimal medium in a 500 mL baffled flask and grown at 30°C with 180 rpm in an orbital shaking incubator.
Aspergillus nidulans defined minimal medium (Barratt et al. 1965), containing (in g l−1) 6 NaNO3, 0.52 KCl, 0.52 MgCl2 and 1.52 KH2PO4 was used in all the A. niger agar plates and liquid cultivations.
