Sentence examples for defined medium for from inspiring English sources

In this study, a serum-free, stroma-free, and chemically defined medium for hematopoietic stem cell (HSC) expansion was systematically developed and optimized using factorial design and the steepest ascent method.

Cells were cultured in defined medium for up to 1 month.

It is very difficult to develop a generally applicable defined medium for these organisms.

Fig. 6A shows an Oct4+ hESC colony cultured in this completely xeno-free defined medium for 7 passages.

I6 cells were continuously cultured in this defined medium (StemPro ESC defined medium) for 6 months on a defined substrate (CellStart).

The mix was incubated for 30 min on a magnetic plate (OZ Bioscience) at 37°C and then replaced by pre-warmed serum-free defined medium for 48 h until fixing in 4% PFA.

In addition, the standard defined medium used for S. cerevisiae, SC or SD medium, is inferior for S. pombe growth to Edinburgh Minimal Medium (EMM) (Gutz et al. 1974), although the basis of this growth difference is not known.

Human hepatic stem cells seeded onto liver biomatrix scaffolds in a hormonally defined medium tailored for adult liver cells lost stem cell markers and differentiated into mature, functional parenchymal cells and remained viable with stable mature cell phenotypes for more than 8 weeks (Wang et al., 2011).

Cells were grown overnight in the same defined medium used for the luminescence experiments and then transferred to fresh medium containing 1000 nM exogenous AI.

To our knowledge, the best chemically defined medium reported for the culture of rat embryos is mR1ECM [34], a phosphate free media used in these studies.

Recent advances in the identification of multiple factors that play a role in sustaining pluripotency have led to the report of several defined medium systems for hESC culture and derivation [5] [9].