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The TP-stained slides were assessed for tumour cells and stromal cells according to the criteria defined in the kit.
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HER-2 immunostaining was performed following the manifacturer's protocol and protein overexpression was determined as defined in the HercepTest kit guide: score 0, 1+, 2+, 3+.
ErbB-2 and ErbB-3 overexpression was determined as defined in the HercepTest kit guide (0 or 1+ negative, 2+ and 3+ positive).
As defined in the HercepTest kit guide, scores of 0 or 1+ were considered negative for HER2/neu overexpression, 2+ was considered weak positive, and 3+ was considered strong positive.
HER2 overexpression was determined as defined in the guide of the HercepTest kit (Dako): scores of 0 or 1+ were considered negative, 2+ weak positive, and 3+ strong positive.
In order to support construction kit development with the help of MBSE in a long term, definitions of such terms as module, platform, type series and construction kit are analyzed and defined in the context of MBSE.
These control samples were tested with the IDEXX kit using the optimal concentrations of sample and conjugate defined in the previous step.
However, crucially for two samples (B2/C3 and B8/C10), the value established by ddPCR is very close to the expected minimum level of methodological sensitivity (for example, 15% for Sanger sequencing, and 5.43% for the p. (G719S) mutation as defined in version 1 of the Qiagen Therascreen kit packaging insert).
The API is defined in Swagger.
The notations are defined in Figure 1a.
The diagrams in the kit were useless.
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