Sentence examples for defined copy from inspiring English sources
HACs/MACs are unique vectors with several advantages: 1) independent maintenance, 2) defined copy number and mitotically stable, 3) no silencing of the transgene, and 4) no limitation of DNA insertion size.
Control plasmids that contain approximately 1000 bases of human genomic sequence with a specific mutation of interest positioned near the middle of the insert and a nearby 6-bp molecular barcode were synthesized, linearized, quantitated, and spiked into genomic DNA derived from formalin-fixed, paraffin-embedded prepared haparaffin-embedded prepared copy number ratios.
Serial dilutions of defined copy numbers of individual qTags were included for each real-time PCR to generate standard curves, which were used to calculate the absolute copy numbers of the variable and common region in each PCR reaction mix.
A standard curve of corresponding mtDNA and nDNA template equivalents with defined copy numbers were included in each run to quantify the content of mtDNA and nDNA in each sample.
Using this approach, cluster separation was only sufficient to allow us to 'bin' a relatively poor percentage of the CNVRs (186 CNVRs, 6.3%) into defined copy number states.
We have constructed an X-chromosome tiling path array using bacterial artificial chromosomes (BACs) and validated it using samples with cytogenetically defined copy number changes.
Thus, short read sequencing cannot capture unique sequence and define copy number.
We define "copy number" of a duplicon as the average number of SDs aligned to each position of the duplicon).
After finding a model with appropriate breakpoints, copy number annotations can be added to the top half of each plot to define copy number status (Fig. 1).
To define copy numbers and diversity of MIBs in macaque species, two consanguineous Mhc homozygous rhesus macaques were chosen for further analysis.
The differences may also be the different analysis algorithms used by the two groups to define copy number and gene expression.
