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After establishing the HIV-1 transmigration assay, we defined conditions that would allow us to examine the enhancing effect of HSV infection of PGEC on HIV-1 transmigration.
Over the last two years we have maintained a colony of NOD mice under defined conditions that develop sialadenitis.
Net expansions of DSR-HSCs occur in vivo, but molecularly defined conditions that support similar responses in vitro are lacking.
We have previously optimised chemically defined conditions that cause hESCs to differentiate as progenitors of endoderm or of mesoderm (Bernardo et al., 2011).
Without Nanog, somatic cell reprogramming does not progress to full pluripotency in chemically defined conditions that support naive pluripotency (Silva et al., 2009).
We also describe defined conditions that permit the isolation of a CD31+ CD45+ EMP, and show that in extended culture on OP9 stroma, that erythroblasts can exhibit enhanced co-expression of both HbF and HbA.
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In a stromal-free defined condition that could drive blood differentiation, we showed that in contrast to WT hESCs, GATA2 −/− hESCs generated a few HPCs (CD34+CD43+) and blood colony-forming units (CFUs) (Additional file 1: Figure S3).
Additionally, a deeper understanding may provide surprising therapeutic angles by defining conditions that allow the controllable reprogramming of endodermal or pancreatic cell populations.
Here we have carried out for the first time a RS M DOEstudy of the influence of these important culture variables and define conditions that maximize biomass production, lipid content (BODIPY® fluorescence) and total lipid production.
This implementation provided rules defining conditions that are limited to existing fragments.
This approach should be applied when the researcher is able to define conditions that must be fulfilled by the candidate gene, e.g. expression in a certain tissue or co-expression with another gene.
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CEO of Professional Science Editing for Scientists @ prosciediting.com