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In this study, the average TEC value of the data between 45° and 70° N was defined as the background TEC.
After preliminary analysis by trial and error, three rectangles that approximately cover the areas with large moment release in Model 0 are defined as the asperities A1, A2, and A3 (gray rectangles in Fig. 3), and the remaining area is defined as the background area (BA).
The multimodal histogram contained one large peak around 1 (defined as the background response) and neurons with fold-changes greater than the first minimum (defined as threshold) were considered responsive.
The entire human proteome was defined as the background.
The remaining fragment end (not classified given other landmarks) was defined as the background.
Starting from a proteome dataset (e.g. Uniprot or IPI) that is defined as the background, an in-silico tryptic digest is obtained.
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Using a 95%% confidence interval, the LOD can be defined as the average background signal plus 2 standard deviations.
FDR was defined as the ratio of background positions in a specific distance to the total number of background positions within the 2000 nt distance.
At this stage we subtract a background fluorescence value from each cell, defined as the median of the background (unsegmented) pixels in the neighborhood of the cell.
The cutoff value was defined as twice the background signal (21 ).
The interaction w ij between two genes i and j is then defined as (9) The background correction step aims to reduce the prediction of false interactions based on spurious correlations and indirect interactions.
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