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Fraction of read aligned is defined as (alignment length + insertions - deletions)/ alignment length + unaligned length - deletions + insertions).
Surgical success was defined as alignment within 10 PD.
At 3 years follow-up, the success rate, defined as alignment within 5 PD, was 77.7%.
In every step, HMM match states are by default defined as alignment columns having less than 5% gaps and minimal information content of 1.2.
Indels were defined as alignment gaps of up to 5 bp, supported by three or more nonredundant pairs of reads and present in at least one-third of reads for autosomic indels or in all of the reads for X-chromosome indels.
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A suboptimal alignment was defined as any alignment which exceeded 3 standard deviations of the mean pairwise genomic divergence (window size 2 kb, slide 100 bp).
A suboptimal alignment was defined as any alignment which exceeded 3 standard deviations of the mean K2 divergence (window size 2 kb, slide 100 bp).
PASA assembles overlapping and compatible alignments into maximal alignment assemblies; compatible alignments are defined as overlapping alignments that are transcribed on the same strand and have identical introns in their regions of overlap.
The alignment coverage was defined as the alignment length divided by the length of the shorter sequence.
An angle of < 180° was defined as varus alignment, and > 180° as valgus alignment.
Suboptimal alignments were defined as those alignments that exceeded 3 standard deviations of the mean pairwise K2 divergences in a sliding window analysis (See Methods), which were removed using a post-alignment filter.
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