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Analysis was performed using the Acknowledge 3.9.1 (Biopac Systems, Inc., Goleta, California) sliding window (delta time) to define peak areas and calculate Haplotype Ratios on an Excel spreadsheet (Microsoft, Redmond, Washington).
The mean background determined from the intensity histogram of recorded image stacks was subtracted [36] and the resulting image stacks were used to automatically define peak regions of interest of synaptic bouton size [37], where AP-evoked fluorescence decrease (FM 1 43 and anti-synaptotagmin-1-cypHer) or increase (synaptopHluorin) occurred in difference images.
Samples were genotyped with the LightTyper software version 1.5 using positive control samples to define peak standards.
For each cluster, we also count the number of tags overlapping with each position and define peak height by the maximum number of overlapping tags.
The additional samples were taken in an attempt to better define peak air and breath concentrations for the THMs during showering.
Comprehensive stage-specific transcriptomic datasets are available and provide an opportunity to define peak transcript accumulation to defined temporal windows of the 46-48 hr IDC [ 10- 12, 62, 63].
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A signal-to-noise ratio of 3 was selected to define peaks, with a maximum of 100 peaks per spectrum.
For peak detection, MZmine requires parameters that define peaks and noise.
Finally, and in contrast to previous approaches, RECLU considers the biological reproducibility to define peaks boundaries.
Some studies define peaks as local maxima in a spectrum [ 2, 11].
This discrepancy can be explained by the different criteria used to define peaks of amplification and SROs.
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