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These criteria are used to define cultured MSCs, however, the exact anatomical locations of these cells in situ remain unclear.
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Differentiation was initiated by placing the cells in mitogen-free chemically defined cultured medium, whose composition is described above.
As shown in the results, NSCs derived from defined cultured hESCs could be frozen and thawed, and NSCs cultured for prolonged periods retain the ability to differentiate into authentic A9 dopaminergic neurons both in vitro and in vivo.
Herein, we describe recent major advances and challenges in propagation of adult SSCs from mice and humans during the past few years, including the use of unique cell surface markers and defined cultured conditions.
Cells were cultured in classic culture medium or in a defined culture medium.
A total of 5 × 10 glioma cells (P7 primary culture) cultured in defined medium were injected subcutaneously into the flank of male nude mice.
Astrocytes and fibroblasts were seeded onto separate PDMS membranes with defined pores, statically cultured in the perfusion culture modules allowing cells to 'cover' or 'bridge' the pores, then perfused continuously with different flow/shear rates (see Table 1) for varying periods of time and monitored using time-lapse phase contrast microscopy.
At current, no Bio-MEMS exist for long-term cultivation and non-invasive quantification of specific cellular responses of adherent individual cells in a small defined cell layer cultured on miniaturized Si3N4 membranes with cell culture areas smaller than 0.2 mm2.
Arthritis was defined as inflammation of the synovia.[ 27] Septic arthritis was defined as bacteria cultured from synovial fluid and/or synovial fluid with WBC count > 50 × 10/L.
We have recently shown that a human colon carcinoma cell line (SW1222) organizes into glandular structures, with well defined polarity when cultured in three-dimensional type I collagen gel.
A patient in whom any of the defined organisms were cultured within 48 hours of ICU admission met the criteria for the composite measure of any resistant organism on admission.
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