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Cognitive performance, mood, self-reported sleep quality, and plasma amino acid concentrations were periodically assessed during a 10-day energy balance period and a subsequent 21 -day, 40% energy deficit period.
Therefore, rather than being tolerant to drought per se, the mutant can overcome a water deficit period more easily than wild type.
For each water deficit period, root and leaf samples were collected from three plants, pooled and frozen in liquid nitrogen before storage at −80 °C.
Total RNA was extracted with the TriZol RNA extraction kit from leaves of single B1K2 and B1K30 plants at the fifth day of water deficit period (Additional file 1: Figure S1).
Comparing free glucose and fructose sugars in clone pair 4607/4608 during the water deficit period, there were significant differences between E+ and E- in the roots; but not in shoots except for fructose at day 5, where E+ shoots accumulated fructose to higher levels than E- shoots.
It has been suggested that three weeks pre-partum to three weeks post-partum is the most energy deficit period for high merit cows [ 25], but the cows in our study were already 40 DIM (days in milk) and might have well passed the critical energy deficit periods, hence the lack of observable treatment differences.
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To avoid excessive vine water stress, supplemental irrigation was used during water deficit periods.
Effective utilization and conservation of water from reservoirs helps to manage water deficit periods.
Monthly temperature and precipitation were evaluated in order to identify relative water surplus and deficit periods and an annual aridity index (AAI) was calculated.
Hay making has been done to bridge this forage deficit periods.
Four additional units are stationed on emergency standby boats that are deployed on demand during water deficit periods.
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