Exact(5)
House Republicans blocked a similar measure in the last Congress and insisted on a much weaker, deficient version of the bill.
A-TLD mre3 cells were transduced with nuclease deficient version of the MRE11 protein that still formed an MRN complex, but the nuclease activity was completely abrogated.
A catalytic deficient version of the plasmid, A3B_Cat, was made by introducing the mutations E68A and E255Q (Burns et al., 2013).
Thus, having generated a mouse mutant that expressed a phosphorylation deficient version of the M3-muscarinic receptor, the question was what impact did this mutation have on muscarinic receptor-mediated physiological responses?
Support for a direct role for mtDNA mutations in aging resulted from the generation of mouse models with a proof-reading deficient version of PolgA; these mice have high levels of mtDNA mutations and develop a premature aging phenotype (Trifunovic et al., 2004; Kujoth et al., 2005; Vermulst et al., 2008).
Similar(55)
Aiming to discriminate the specific relevance of the interaction of the IFNα/βBP with GAGs at cell surface during poxvirus infections we generated mutant VACV and ECTV expressing the GAG-binding deficient versions of the IFNα/βBP.
These sequences were inserted into a movement-deficient version of CPMV RNA-2 which is rescued from cowpea plants (Vigna unguiculota) by inoculation with RNA-1.
We then determined the frequencies of recombination for ade6 alleles in strains expressing a phosphorylation-deficient version of Atf1 (Atf1-11M-HA) [40].
An ORF27-deficient version of the eGFP-ORF58 tagged virus was made by a second round of shuttle mutagenesis, introducing stop codons into an ORF27 genomic ScaI site as described [9].
The prolonged phase of insulin release was, however, significantly diminished in β-cells isolated from mice expressing the phosphorylation-deficient version of the M3-muscarinic receptor [ 44].
A scrambled version of the BH4 domain of Bcl-2 (BH4-Bcl-2SCR) and a 'binding-deficient' version of BH4-Bcl-2, in which the surface-accessible residues were altered (BH4-Bcl-2BIND), were used as negative controls.
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