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The alteration in the formation of [14C]glucose-labeled lipids was not caused by a deficient uptake of glucose because we observed no changes in the rate of [3H]deoxyglucose uptake in cells treated with CVT or vehicle (Figure 4D).
The phospholipid hypothesis suggests that deficient uptake or excessive breakdown of membrane phospholipids or changes in membrane phospholipid composition may be associated with schizophrenia [ 3].
A deficient uptake has been implicated in the pathogenesis of ischemic brain damage [ 11] and may be involved in neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) [ 12].
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In this regard, our analysis suggests that a neural population may counteract the effect of excess extracellular glutamate resulting from deficient astrocytic uptake only if the value of (frac {m_{G}^{P}}{m_{G}^{I}}) lies in a well-defined interval ([I_{1},I_{2}]) which depends on inherent biophysical and cellular properties of the population under consideration.
Deficient cellular uptake of drug was excluded as a cause of resistance.
We have found that similar astrocytes in tubers have deficient glutamate uptake [ 34].
sat4 / hal4Δ hal5Δ double mutants also exhibit cation sensitivity and deficient K+ uptake (Pérez-Valle et al., 2007).
trk1Δ trk2Δ mutants are sensitive to toxic cations including hygromycin B and show deficient K+ uptake (Madrid et al., 1998, Navarrete et al., 2010).
One hypothesis is that deficient glutamate uptake could be one underlying factor leading to increased mGluR5 expression in astrocytes across the epileptogenic hippocampal formation, that is, in HS cases.
Perhaps the benefits of a higher calcium intake associated with a reduced risk of colon cancer detection (Wu et al, 2002) can be related to a compensation of deficient calcium uptake in patients with impaired VDR signalling after aberrant activation of Snail1 as part of the transformation process.
While others have also discovered that membrane traffic mutants are sensitive to hygromycin B (recent examples include [ Banuelos et al. (2010), Dudley et al. (2005), and Conboy and Cyert (2000)], the novel aspects of this work are the findings that many of these mutants show deficient K+ uptake and that some phenotypes can be suppressed by the addition of excess K+ in the medium.
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