MCF-7/MR cells grown in a riboflavin-deficient medium to avoid its intense intravesicular green fluorescence [12], were incubated with topotecan (5µM) in the presence or absence of the specific ABCG2 transport inhibitor FTC.
The three mutations found in the PSC patients were introduced into hMYH expression plasmids and the mutant proteins were expressed in a mutY deficient E.coli strain to avoid contamination of endogenous MutY.
Since the xenograft models utilize immune deficient mouse strains to avoid graft rejection, immunologic microenvironments of transplanted GBM cells can be specifically modified by choosing a recipient mouse strain with defined immune deficiency status.
In initial experiments, we infected mice with latency-deficient MHV-68 [35] to avoid the risk of persistent virus contaminating the hybridomas.
A range of potential bridge retrofit measures may be used to mitigate seismic damage in deficient bridges, and help to avoid associated social and economic losses.
To further evaluate the differential effects of iNKT cells on anti-DNA versus anti-CL antibodies in vivo, we reconstituted BALB/c SCID mice with purified B cells from iNKT cell-deficient Jα18° BALB/c mice (to avoid any contamination with iNKT cells or iNKT cell-exposed B cells).
To explore this, we studied hair follicle development in keratinocyte-specific TAK1-deficient (Map3k7fl/flK5-Cre) mice and subsequent hair follicle cycling in tamoxifen-inducible keratinocyte-specific TAK1 deficient mice (Map3k7fl/flK14-Cre-ERT2) to avoid gene-targeting in embryonic development because this might damage the hair follicle, impairing its later capacity to cycle.
The present study was designed to determine the effective chemical forms of selenium and their optimal levels on T-cell mitogenesis with splenic cells from mice given a selenium-deficient diet for 8 weeks to avoid effects of cellular selenium sources.
Since this code diversitya is able to avoid a rank deficient matrix, similar performance of ZF receivers compared to ML can be expected even for SNR that is not so high.
This adjusted value is used to calculate various cutoff levels to avoid having severely deficient values skew the population median.
In order to avoid a rank deficient-induced channel matrix, in [9], it is proposed that the phase of the channel gains be modified as h j, i → h j, i × e j Φ i, (3).
