12, 18, 28 The cells devoid of complex IV activity and with complex II (respiratory-deficient) are stained blue by the histochemical technique as opposed to the cells with complex IV activity, which are stained brown (Fig 1A C).
Following a 48 h fast, numerous cellular inclusions were noted in the PanK1-deficient livers, and staining with oil red O clearly showed the accumulation of neutral lipid droplets (Fig. 4B).
"Reduced staining" included totally deficient fibers, partially deficient fibers, and fibers with hypodensity of mitochondria; the involved area was semi-quantified as variable focal (<25% of the tissue area), variable regional 25-755% of the tissue area) and widespread (>75% of the tissue area).
In a prospective cohort study on patients with clinically significant aqueous-deficient dry eye, increased staining in the corneal inferior zone was found in patients with primary SS.
However, approximately 50% of BLM-deficient cells, 80% of WRN-deficient cells and 60% of RECQL4-deficient cells still stained positively for SA- β-gal when grown in 3% oxygen.
Furthermore, all abnormal nuclei in UPF2 deficient fetal livers stained positive for the mitotic marker pHH3, strongly suggesting that these cells are either arrested or at least retarded in mitosis.
To investigate synaptic changes within the thalamocortical system of Ppt1 deficient mice, we stained sections from 1 (pre/early-symptomatic), 3, 5 and 7 (late-symptomatic -month-old controlate-symptomatic -month-oldange late-symptomatic -month-old
GFP-Spire1CΔKIND: A Cos7 cell overexpressing the formin-binding deficient GFP-Spire1CΔKIND stained with phalloidin-568 reveals significant accumulation of actin on mitochondria compared to control cells (Mander's: 0.55 ± 0.017, ncells = 18, p < 0.05).
As expected dectin-1 deficient mice did not show staining for dectin-1 in the intestine (data not shown).
Finally, we were unable to find any platelet cluster in wild-type or Asm-deficient lungs in vivo after staining with anti-CD41 antibodies.
