Fertilizing a calcareous soil with an Fe-deficient solution increased Cu accumulation by roots and shoots in two wheat cultivars (Chaignon et al.2002).2002
In this study we cultivated rice plants in Fe-sufficient hydroponic solution for 5 weeks before transferring to Fe-deficient solution for a period of 1 week prior to sampling.
Inoue et al. (2003) exposed very young seedlings to Fe-sufficient nutrient solution for 2 weeks, whereas Kobayashi et al. (2009) cultivated seedlings in Fe-sufficient nutrient solution for 3 weeks, before transferring plants to Fe-deficient solution for 1 week.
In human volunteers, infusion of 5-HT or its precursor reduced cough responses to a chloride-deficient solution [ 16].
After 2 weeks, the seedlings were transferred to a Zn-deficient nutrient solution for 3 weeks and harvested for analysis.
To investigate the role of polyaniline (PANI) in the corrosion protection of stainless steel (SS) in oxygen-deficient acidic solution, a separate doped PANI film electrode on a glass substrate was prepared and the test solution (1 M H2SO4) was purged with high-purity N2 until dissolved oxygen level decreased more than two orders of magnitude.
To identify additional responsive genes, we transferred rice seedlings at the six-leaf stage to an N-deficient hydroponic solution.
Plants in Lot 6 received 100 µM SNP plus 100 µM methylene blue (MB) (an NO scavenger) in Fe-deficient nutrient solution.
Plants in Lots 3, 4 and 5, respectively, were supplied with 10, 50 or 100 µM sodium nitroprusside (SNP), an exogenous NO donor in Fe-deficient nutrient solution.
The number of genes with GO ID column is the number of genes differentially expressed between IsoClark plants grown in iron sufficient and iron deficient hydroponics solutions with the corresponding GO Slim ID.
Nine days after being placed in hydroponics, the roots of all seedlings were rinsed six times in diH2O and transferred to either Fe sufficient or deficient nutrient solutions (100 μM vs. 50 μM Fe(NO3 39H2O).
