The finding that MMR-deficiency does not alter 5-fluorouracil cytotoxicity in a p53-deficient setting may be of clinical interest for the treatment of colorectal cancer, despite the previously reported in vitro association of MMR with resistance to 5-fluorouracil in p53-proficient cells (Carethers et al, 1999).
Given the upper bound for the number of deficient members, we consider uncertainty in the locations of deficient members, i.e., the set of deficient members is not specified in advance.
Based on the seven deficient items, a set of nine system design requirements has been suggested in this work.
These characteristics included that MSCs should be adherent to plastic when maintained in standard tissue culture conditions, be able to differentiate into osteoblasts, chondroblasts, and adipocytes, and also express or be deficient in a set of panel markers [ 32].
Due to duplicated arrays in different experiments (from one cancer entity) and several deficient arrays, the set of arrays used in the analysis is smaller than all available arrays.
For the generation of autophagy-deficient CT26, a set of plasmids encoding shRNAs specific for murine Atg5 plus a control shRNA were obtained from Origen and used as described above.
To generate a more comprehensive picture of the mutation spectra arising in MMR-deficient tumors, and in particular, to interpret their clinical relevance with respect to diagnostically assessing MSI and therapeutically targeting MMR-deficient tumors, we sequenced another comprehensive set of MMR-deficient tumors.
To formally test whether the combination of FK866 and olaparib could increase the therapeutic window in a HR-deficient setting, we assessed the effect of FK866/Olaparib on isogenic DLD1 BRCA2+/+ and BRCA2−/− cells.
The nature of the T-cell response in wild-type versus COMP-deficient animals requires another set of experiments as the circulating COMP affects T-cell tolerance, and we therefore need to determine the major T-cell epitopes.
