The lack of exons 2 and 27 leads to a deficient protein sequence in the Ig1 region and the short cytoplasmic tail.
To test the export activity of the identified candidate NES in BRMS1, we generated a construct encoding 18 residues (74LKEKLFRERLSQLRLRLE91, hydrophobic residues are underlined) inserted between the export deficient Rev1.4 sequence and eGFP (Figure 5A), rendering the pRev1.4-BRMS1-eGFP plasmid.
Positions deviating from the idealized correlation are shown to be deficient in sequencing coverage in either the single or pooled sequencing (Additional file 1: Figure S3).
Female gametes were also deficient in sequences related to DNA metabolic processes (e.g., protein-DNA complex assembly, nucleosome organization) as well as microtubule-based movement and male gametes had underrepresented genes in the cellulose binding group.
A S4(13 -PV analogue, S4(13 -PVscr, displaying a scrambled amino acid sequence, deficient cell internalization and drug delivery inability, was used in this study for comparative purposes.
The percentage of unisequences with homologues was similar between BasidiomycotaDB and UniProtKB, indicating that the latter is not deficient in Basidiomycota sequences (Table 2).
We searched first for G + C-rich (≥61%), CpG deficient (≤1/100 bp) sequences of ≥500 bp in length and identified 1954 examples.
ath, acyl-protein thioesterase; spe, spermatogenesis-deficient. A sequence alignment of the primary sequence of the 15 C. elegans DHHCs confirms that all of them contain the DHHC motif required for enzymatic activity within a cysteine-rich domain.
This result corresponds well with the observation that MMR genes in natural bacteria isolated from stressful environments are often deficient or show high sequence mosaicism [ 33– 33].
Similar sequences were prevalent in the upstream, intronic, and downstream sequences, but deficient in exons (Table 1).
