For this investigation, we employed yeast two-hybrid assays (Fields and Song, 1989), in which a positive protein-protein interaction enables transformed yeasts to grow on the nutrient-deficient selection plate.
One possible explanation for this difference is that in the mismatch-deficient M. tuberculosis, selection against unstable sequences is stronger than in E. coli, and that this results in a stronger stabilization of the genome by context-dependent codon choice.
By transduction of target cells harboring engineered recognition sites for the yeast Flp recombinase with integration-defective lentiviral vectors containing an ATG-deficient hygromycin B selection gene we demonstrate precise integration of lentiviral vector-derived DNA circles in a drug-selective approach.
Thus, 'a frontal neuronal circuitry, underlying saccade direction selection is deficient' (Rieger et al., 2008).
33 Also, in vitro data on aspirin are conflicting: it does not improve replication fidelity, 22 but was reported to reduce the accumulation of microsatellite mutations in MMR-deficient cells by genetic selection.
However, it might play a role in acquired resistance due to selection of MMR deficient cells during platinum-based chemotherapy, but this needs further investigation.
This enrichment was by itself insufficient, so the thioxanthine population was further enriched using the "suicide selection" for IgM deficient mutants [ 14].
Given that these signaling elements play an important role in central B-cell tolerance, however, it is not entirely clear to what extent the increased levels of autoreactive B cells after the peripheral checkpoint reflect a defect in peripheral selection per se in MyD88-deficient, IRAK4-deficient and UNC93B1-deficient patients or whether they are a consequence of defective central tolerance.
TEPW transduction led to a complete surface expression of the GPI anchor and CD59 in PIGA-deficient cell lines without any selection procedure.
SD uracil-deficient medium was used for selection of strains with URA3 marker.
