In some cases, if you didn't blow enough air into a machine you get what is called a "deficient sample," which is qualified as a refusal.
Compared with traditional method, SVM can be established under condition of deficient samples and abnormal observation result can be rejected effectively.
This method can overcome the biased assessment from the deficient samples between positives and negatives.
Deficient samples could be distinguished from normals by a lower bound fraction, while τ bound was similar for most samples.
The opposite was true for P deficient sample and about 2.5 times more transcripts were up- than down-regulated.
In capillary blood, although the ability to detect deficient samples increased, the number of invalid results also increased.
The 19 deficient samples identified by BinaxNOW are identical to the 18 samples shown below the 30% cutoff with the reference assay plus the 1 additional sample directly above this cutoff.
For the amplification protocol, 0.5 μg and 0.1 μg samples from the same animal were processed together for each step, again with balanced numbers of normal and vitamin deficient samples processed together.
However, all 58 hMLH1 deficient samples were analysed by direct sequencing without a prior screening step, and only three of these samples harboured a CTNNB1 mutation, indicating the low frequency of such mutations.
Two of the deficient samples were characterized as type 2 and likely type 2 on the basis of radioimmunoelectrophoresis showing a decrease in large multimers (confirmed type 2) and very low ristocetin cofactor activity values (suspected type 2).
