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Sporozoites, deficient in expressing P36p resulted in aborted development in hepatocytes, prior to parasite replication.
In this study, we therefore generated P. falciparum parasites that were deficient in expressing P52 (PFD0215c), the equivalent of P. berghei P36p.
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Previous reports have shown that Nod2 can protect cultured cells from bacterial infection and this activity is deficient in cells expressing the 3020insC polymorphism [10].
This capability allows Treg activity to be directed to specific tissue microenvironments and target tissues deficient in APCs expressing MHC class II molecules.
For this purpose, we constructed strains deficient in Pep4p expressing the empty vector control, and equivalent levels of FLAG-tagged wild-type Pep4p, a double-point mutant form deficient in proteolytic activity, and human CatD.
In rodents, female mice deficient in Paternally expressed gene 1/Mesoderm specific transcript (Peg1/MEST) [10] or Paternally expressed gene 3 (Peg3) [11] have aberrant maternal nurturing behavior with low incidences of pup retrieval, nest building, and milk ejection.
Using cells deficient in PSs expression, expressing human wild-type PS1, or PS1 familial AD FADD) mutants, we found that PS1 affects mitochondrial energy metabolism (ATP levels and oxygen consumption) and expression of mitochondrial proteins.
Furthermore, Roger and colleagues found that mouse macrophages genetically deficient in MIF expressed significantly lower levels of TLR4 protein [30].
After mosaic deletion of aPKC due to a single low dose injection of tamoxifen at P1, c-Myc signal was significantly reduced in aPKC deficient cells expressing the EYFP Cre reporter compared to that of non-recombined control EYFP negative cells within the same retina (Supplementary Fig. 1d, e).
Moreover, lymphoma formation was rapidly accelerated in p27 deficient mice expressing the dominant oncogenes myc (Eμ-myc mice) or cyclin E [48], [49].
10.7554/eLife.01741.009 Figure 5. ABA import by AIT1/NRT1.2 in protease deficient yeast expressing ABACUS1 variants.
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