If, let's say, he was working on a screenplay and somebody came in and said, "Your mother has just died, David", he'd say, "Look, please don't worry me at the moment, I'm much too caught up in this".' This makes for a deficient human being, but if we expect great art, sometimes we have to live without the social niceties.
This may explain our observations of altered mitochondrial morphology in PINK1 deficient human neurons (see below).
The construct was transfected into the HLA class I deficient human B cell line 721.221 as previously described [37].
Following treatment with a DNA damaging agent, VER-150548 appeared no longer able to induce reduplication and polyploidy in p53 proficient or deficient human carcinoma cells.
This resulted in a DNA vaccine (pSG2.HAV), a recombinant MVA vaccine (MVA.HAV) and a recombinant replication deficient human adenovirus type 5 vaccine (Ad5.HAV).
Transient expression of cloned MHC class I cDNA was achieved by electroporation of plasmid DNA into the MHC class I deficient human B-cell line 721.221 [66].
Jin et al. showed very recently that RecQ4 deficient human cells have increased sensitivity to HU, camptothecin (CPT) and doxorubicin (DOX), modest sensitivity to UV or ionizing irradiation[27].
Binding of rCR2 to C3d+ cells was also evaluated by flow cytometry, whereby human C3d+ RBCs were generated by exposing sensitized sheep red blood cells to C6 deficient human sera.
Secreted human CD1d molecules (sCD1d) were generated by truncating the transmembrane and cytoplasmic domains, and were expressed in the HLA class I deficient human lymphoblastoid cell line 721.221 [24], [24].
A498 and 786-O, two VHL deficient human RCC cell lines[20], were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and cultured for less than one month with aliquots then frozen.
