As far as the biomedical application is concerned, traditional aliphatic polycarbonates usually suffer from the strong hydrophobicity, deficient functionality, and insufficient compatibility with cell/organs.
The cells that did not express BRCA1 had reduced RAD51 foci formation upon DNA damage, suggesting a deficient HR functionality (Fig. 3b,c).
Well-carbonized BC samples, on the other hand, were deficient in surface oxygen functionality even after the PPAO treatment.
The present study is based on assessments performed in those studies, where functionality of each variation was evaluated in comparison to the performance of the corresponding wild-type (wt) protein and functionally deficient negative controls.
First, we directly introduced the carboxyl acid functionality onto the electron deficient polyester using Huisgen 1,3-dipolar cycloaddition protocol in N,N-dimethyl formamide at 40 °C for 16 h.
However, recent studies have directly investigated the contribution of Agrin to GBM functionality by using mutant mice deficient for Agrin expression specifically in podocytes [ 41- 43].
Additional deletion of Ink4a/Arf (encoding p16Ink4a and p19Arf) or Trp53 (encoding p53, a downstream target of p19Arf) in a Cited2-deficient background restores HSC functionality and rescues mice from bone marrow failure.
In other experiments involving the repression of miRNA functionality using 'antagomirs' [5] and miR-155 deficient mice, DIANA-mirExTra has correctly identified the repressed murine miRNA in both occasions (mmu-miR-122a and mmu-miR-155) using microarray data.
It is plausible that the H2SO4p-dopes the SWCNTs and the attachment of chemical functionalities helps to stabilize p-doping owing to their electron-deficient property.
To evaluate functionality, we extensively tested the VFP-tagged Gq in MEF cells deficient in Gq/11.
Interestingly, MuRF-1 deficient mice exhibit no major alterations in the levels of ubiquitination within the myocardium, suggesting redundant functionality among MuRFs in cardiac muscle [ 157].
