8 More recently, in Haiti, its sensitivity for detecting deficient subjects with activity below 30% of normal was estimated to be 84.8%.
To determine the accuracy of a pentaplex PCR system for detecting MMR deficient CRCs, we investigated a cohort of 213 CRCs which comprised of 114 MMR-deficient and 99 MMR-proficient tumors.
But this improvement was only due to increased sensitivity for detecting MSH6-deficient tumors (62.5%; 5 of 8 CRCs), without any associated change in sensitivity for identification of MLH1 (96.0%), MSH2 (100%), and PMS2 (100%) deficient (Figure 2B).
Hence, the optimized pentaplex assay is highly specific and sensitive for detecting MMR-deficient CRCs, and that the availability of normal DNA from a CRC patient does not necessarily enhance its performance.
We provide evidence that an optimized pentaplex PCR assay should be the preferred method for MSI evaluation in clinical and research laboratories, as it is rapid, economical, highly sensitive and specific for detecting MMR-deficient CRCs and obviates the need for reference normal DNA.
The PAB antibody seems to be appropriate for detecting cell-wall-deficient P. acnes because an epitope of lipoteichoic acid detected by this antibody is more exposed in cell-wall-deficient forms than in conventional forms of the bacterium.
Unfortunately, although multiple studies have repeatedly shown that mononucleotide MSI markers offer higher accuracy for detecting MSI-H or MMR-deficient tumors [10], this approach has not gained sufficient recognition or acceptance.
In a Russian population with high levels of alcohol consumption, carbohydrate-deficient transferrin (CDT) might be the most sensitive and specific biomarker for detecting ethanol consumption above 40 g/day.
Strategies for detecting liars.
The oligonucleotide used as a probe for Northern blotting for detecting 47S rRNA was described previously [29] and is illustrated in Figure 1A. Figure 1B shows that the 47S rRNA level was decreased in 1A6/DRIM deficient cells (left panel).
