For the Ca2+-deficient experiments, sperm were examined in HS0Ca or HSB0Ca buffers (buffer HS or HSB prepared without added CaCl2).
Iron-deficient diet experiments were as follows: 8-week-old male C57BL/6Crl wild-type mice were fed an iron-deficient diet (ssniff EF R/M Iron Deficient; Charles River, Calco, LC, Italy; iron content, <10 mg/kg) for 9 days before death, or for 6 days before the 24- to 48-hour starvation period.
F1 progeny were then intercrossed to generate PTPN4-deficient mice for experiments.
Resulting PTPN4/PTPN3 double heterozygotes were then intercrossed to generate double-deficient animals for experiments, or were crossed to ptpn13 ΔPTP/ΔPTP (C57BL/6 background) mice also described previously [25].
As a case-study, the baseline regulation and activity of LDLR were considered under lipoprotein-deficient conditions, from experiments described in Figs 1 3.
This is in contrast with PHA-producing enrichment cultures, which were reported to achieve higher storage contents during nitrogen-deficient fed-batch experiments [ 45].
This intriguing observation was confirmed by qRT-PCR analyses that revealed a ~30-fold induction in adult, iron-deficient rats, including experiments performed with 2 different sets of SP6-specific primers.
If the question was posed in more general terms, such as running experiments with deficient methods, failing to report deficiencies or misrepresenting data, the straight average suggested that 46% of researchers had seen others get up to such shenanigans.
In order to determine whether presence of TLR3 on macrophages or DCs was sufficient to decrease the susceptibility of a TLR3 deficient host, adoptive transfer experiments were performed.
Dialysed serum was used for all experiments with deficient media.
We observed declines in the frequency and self-renewal of Imp1 deficient neurospheres in both experiments.
