Absence of RSPH4A due to mutations in RSPH4A results in deficient axonemal assembly of the radial spoke head components RSPH1 and RSPH9.
Since M. tuberculosis cells growing in macrophages and those exposed to cephalexin antibiotic are deficient in FtsZ assembly [5], [26], we examined if ClpX is involved in regulating FtsZ assembly and cell division in M. tuberculosis.
In contrast, the ∆flgE mutant deficient in hook assembly did not seem to affect MakA secretion (Fig. 2a, lane 6, lower panel).
Together with assembly deficient Rev-dependent HIV-1 Gag in mouse cells, this model can provide a valuable tool to study how HIV assembly is temporally and spatially regulated and coordinated with genome packing.
Interestingly, analyses of the transfected cellular lysates indicated similar levels of intracellular Gag accumulation in cells transfected with any of the three assembly deficient Gag constructs, evidently reflecting similar levels of Gag expression regardless of the specific Gag construct (Figure 2C, compare line 3 5 and lines 1).
As the authors note, it is surprising that mutants that are self-assembly deficient are remarkably efficient in de novo centriole formation, but this does not necessarily prove the self-assembly model wrong.
Purified proteins may be self-assembly deficient in vitro, but be assisted by other proteins (restoring some oligomerization) in vivo.
These results suggest that in LPCAT3-deficient mice, the assembly of TG into lipoproteins is normal until some degree, but becomes severely inhibited when high amounts of TGs have to be transported.
The results argue that viral envelope protein RGV 53R can be silenced and the virions assembly was deficient by amiR-53R-1, and further identified the first amiRNA of envelope protein gene from iridovirus that was able to cause resistance to virus infection in fish cells.
The formation of P-bodies in yeast was not affected in mutant strains that were deficient in stress granules assembly.
