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Thus, we chose to analyze spg242 over the deficiency line Df 3R 3450, which removes the spg locus [53].
Ppt1 LOF mutant alleles Ppt1A179T, Ppt1S77F, and the Df(1 446-20 deficiency line were provided by Dr. Robert Glaser (Wadsworth Center, NYS Department of Health, Albany, NY).
That is R cells in the Df 2R Dg248 deficiency line are substantially more disrupted when compared with Dg point mutants.
However in both Dg point mutants in the deficiency line, Df (2R) Dg248, we were unable to detect any differences between mutants and wild-type eye discs in the expression of neuronal markers or in cell polarity.
Similar accumulation of Ci-155 was also observed in the clones of hibΔ6, which was a null mutant of rdx isolated by Jiang's group, and also in the clones of Df 3R Exel6171, a deficiency line [41].
Furthermore, defects in the external eye (lens, bristles) are attributable to lack of mRpL34 as transgenic expression of this gene in the deficiency line rescues the lens and bristle organization (Fig 7E) though the R cells are somewhat disorganized (Fig. S3).
Similar(42)
Mod4 was mapped by following its lethality phenotype in crosses with deficiency lines.
The control and all the deficiency lines have an isogenic background except for deletions.
A collection of 216 deficiency lines were screened for modifiers of act-Gal4:UAS-DysN-RNAi/CyO mutant.
We therefore obtained several loss-of-function mutants or deficiency lines of drp1, opa1 and marf and generated UAS-drp1, UAS-opa1 and UAS-marf transgenic lines.
A kit of ∼400 deficiency lines for which homozygotes have a recognizable nervous system and intact body walls encompasses >80% of the genome.
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