Deficiency cut-offs were Se<0.1 μmol/L and Zn<9.9 μmol/L.
We reported definition of vitamin D deficiency and cut-off of 25(OH D.
Although there is no global consensus on the biochemical definition of vitamin D deficiency, a cut-off point of ≤ 37.5 nmol/L (≤ 15 ng/mL) 25(OH D is typically used to determine deficiency [ 13].
Despite the variation in measurements, all were appropriate to classify insulin deficiency and cut-offs were largely comparable with most approximating 0.2 nmol/L, 21 22 24–26 28 30 and four studies using a slightly more conservative cut-off (0.03 0.08 nmol/L).
52 According to the US Endocrine Society, which addresses the evaluation and treatment of patients with specific diseases who are at risk for vitamin D deficiency, a cut-off level <20 ng/mL (50 nmol/L) for 25(OH D defined vitamin D deficiency.
In the 5-year observation period every week, healthy blood donors (overall n = 268) were tested for GPI deficiency to establish cut-off values (mean + 2 SD) for GPI-deficient cells.
Even a clear-cut deficiency of one or more ETC activities in vitro does not prove a genetic defect of oxidative phosphorylation because ETC deficiencies can be secondary to other conditions [41], [49] [51].
For predicting insulin deficiency, the best cut-offs for age at diagnosis were <30 years (2 studies) or ≤40 years (4 studies).
Diagnostic accuracy studies of any routinely available clinical predictors against a reference standard of insulin deficiency defined by cut-offs of C-peptide concentrations.
A way to at least partly overcome these deficiencies is to cut off all rare species or weak interactions below a frequency threshold [ 3, 9, 33, 34] or to control for sampling effort in null models [ 7, 8, 13, 19, 25, 35].
Thus, we first analyzed the predefined endpoints in relation to MBL levels, and only in a secondary analysis chose a threshold of ≤500 ng/mL for the definition of MBL deficiency, as this cut-off has been shown to reliably predict low-producing MBL2 genotypes [ 23].
