To examine any defect in the polarity determination, we stained ovaries with dorsal marker Gurken.
Straight et al. [ 33] ablated expression of PALS1 in MDCK cells in a cyst formation assay, and that led to defects in polarity determination and the failure of cysts to properly form a lumen.
The assessment provides, for example, information on impurity levels derived from cathodoluminescence spectroscopy, analysis of dopant concentra tions at a level that, in some cases, is several orders of magnitude lower than that attainable by x-ray microanalysis, the mapping of defects, and the determination of carrier lifetimes and the charge carrier capture cross sections of impurities.
However, an examination of development in Map3k1-deficient XY embryos on this genetic background revealed no significant defects in testis determination, although minor abnormalities were observed, including an increase in gonadal length.
Defects in situs determination are rare, but when they do occur they are particularly associated with congenital heart disease (CHD) [ 2].
Overall, these data indicate a delay in Sry expression reaching peak levels in Map3k4 tm1Flv/+ and T hp/+ gonads on B6.YAKR, likely accounting for defects in testis determination in these strains.
Notably, an XY embryo lacking p38α alone did not exhibit overt defects in testis determination, while embryos lacking both copies of p38α and only one of p38β did exhibit gonadal sex reversal (data not shown).
The frequencies of these mislocalization phenotypes were similar to that of defects in cell-fate determination after the S4 divisions in ipla-1 mutants (62%; Figure 2I).
The presence of vacancies or root defects in the crystal structure is confirmed by determination of a pycnometer density of CuCl2 sample.
Mutations in the Drosophila PAPST1 gene, slalom, are associated with defects in multiple signaling pathways, including Wnt/Wingless (Wg) and Hedgehog (Hh) signaling, and in the determination of the embryonic dorsal/ventral axis [30].
