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The phenotypes shown in Fig. 3 make it clear that all three double homozygous mutants exhibit even stronger and more distinct developmental phenotypes than any single PRF1, PRF2, or PRF3 defective plant.
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The use of conditional ABP1 defective plants reveals that the protein is essential for maintenance of the root meristem and acts at least on the D-type CYCLIN/RETINOBLASTOMA pathway to control entry into the cell cycle.
The dwarf phenotype also became more severe with later generations containing smaller, more defective plants.
It therefore makes a lot of sense that potassium is an element that cannot be kept at sufficiently high levels in Casparian strip defective plants.
This screen yielded 9 adhesion-defective plants, two of which (cals5-3 and cals5-4, Ws and Landsberg ecotypes, respectively) had severe pollen surface defects apparent with light, scanning, or transmission electron microscopy (Fig. 1).
E.g., the negligence of an Army surgeon during an operation in sewing up a towel in the abdomen of a soldier; and negligence in quartering a soldier in barracks known to be unsafe because of a defective heating plant.
Further genetic studies suggested that the defective T1 plants are likely homozygous for the pOsSRT1::OsSRT1-antisense transgene as they failed to segregate out normal plants in 4 subsequent generations.
The most severe fer-4 seedlings do not survive, but the same range of phenotypes bled true in progeny from less severely defective mutant plants.
An enhanced responsiveness of root hairs to rhizobia was observed in several infection-defective leguminous plants [ 28, 60- 62], which could suggest the presence of a mechanism that represses root cell competence in wild type plants [ 61].
Mutation of SP1 results in plants defective in panicle elongation, thus leads to the short-panicle phenotype (Li et al. 2009).
Here, we report characterization of a mutant isolated from a genetic screen in Arabidopsis thauana designed to identify plants defective in degradation of an Aux/IAA fusion protein, Aux/IAA1-luciferase (IAA1-LUG).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com