We examined whether this defect could be attributed to defective expression of Notch on T-PDK1−/− DNs.
In S. cerevisiae and S. pombe, the defect has been attributed to defective expression and trafficking of the Can1 and Cat1 basic amino acid transporters (Aspuria & Tamanoi, 2008; Urano et al, 2000).
Similarly, CD4 T cells expressing a dominant-negative mutant of CREB showed defective expression of IL-2, IL-4, and IFN-γ.
Considering the limited number of genes deregulated in SCNT embryos, one may suggest that a simple defect in the expression of a few genes may translate into defective expression of a great number of genes in a ripple effect.
Defective expression of cyclins and aurora B kinase might underlie the cell cycle defects of NFAT5−/− cells under hypertonic stress, since cyclins A2 and B1 are needed for S and G2/M progression in different cell types [44], [45], and aurora B kinase is a positive regulator of cell cycle progression through G1/S to M in T cells [41].
Defective expression of SMARCD2 leads to transcriptional and chromatin changes in acute myeloid leukemia (AML) human promyelocytic cells.
Defective expression of E-cad in methylated cell lines was markedly recovered after treated with 5-Aza-dC (5-aza-2′-deoxycytidine).
The defective expression of some of these E3 ligases has been related to the development of autoimmune disease, in experimental murine and human models, remarking its possible pathogenic role.
Thus, promoter-hypermethylation of E-cad is significantly associated with its defective expression and tumor differentiation, and the demethylating observation proposes a therapeutic strategy to reverse the tumor's malignancy by restoring normal expression of E-cad.
The defective expression of RFT1 increases the heading date to more than 200 days, while, RFT1 overexpression reduces the heading date (Komiya et al. 2008; Komiya et al. 2009).
RFT1 is a major floral activator under LD conditions, and its defective expression increases the heading date to above 200 days (Komiya et al. 2009), while, RFT1 overexpression reduces the heading date (Komiya et al. 2008).
