This defect in specification suggests that FoxA is required for coordinating the differentiation of cell types comprising the regenerating pharynx.
Although the Le-cre; Wls fl/fl mouse only shows reduced numbers of RPE cells, not a defect in specification, this might be explained by developmental stage, as expression of Le-cre occurs post-induction.
We confirmed that reduced βPS localization at MTJs in gyc76C mutant embryos was not due to defects in specification or differentiation of tendon cells to which the dorsal muscles attach by staining for Stripe (Sr) and Held Out Wings (HOW) which label tendon cell precursors and differentiated tendon cells, respectively (Becker et al., 1997; Nabel-Rosen et al., 1999).
Given that Lhx1 is first detectable at E11.5 when SCN neurogenesis begins, we initially hypothesized that Six3-Cre; Lhx1lox/lox mice would have an early SCN specification defect, akin to neuronal progenitor-specific deletion of Six3 (VanDunk et al., 2011) or constitutive deletion of Six6 or Lhx2 (Clark et al., 2013; Roy et al., 2013), rather than a terminal differentiation defect.
In the CBR system, each case is represented using the design specifications, defect items and corresponding costs.
Walkthroughs and inspections are used to detect a requirements defect at a specification time.
Finally, as in lsy-2 mutants, mutations in SUMO-recruited Mec components cause the ASE asymmetry specification defect.
These were identified by considering the common defects of requirements specifications, and tracing back their potential causes within crime scripts.
Therefore, Mec complex mutants also exhibit the ASE asymmetry specification defect, further supporting the idea that lsy-2 is genetically related to Mec class genes.
Further we find an initial defect in neuronal specification of triple null NS cells after one week of differentiation.
