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To test whether the crHyb-binding defect arises from DNA in the guide region or the cr repeat region, we measured the binding dynamics of the Cas9 tracrRNA crD.Hyb complex, (Fig. 3a).
This defect arises from the absence of a term in the third moment, the equilibrium heat flow tensor, proportional to the cube of the fluid velocity.
Cloning and sequencing of complementary DNA's from normal and WHHL rabbits, shows that this defect arises from an in-frame deletion of 12 nucleotides that eliminates four amino acids from the cysteine-rich ligand binding domain of the LDL receptor.
Most interestingly, even in this case the defect arises from failure to turn-off cGMP production.
It is likely that the T cell maturation defect arises from the cumulative effects of relatively small gene expression changes within the Zfp335 regulatory network, possibly resulting in mild perturbations of multiple pathways whose functional consequences may not be individually significant but in combination may negatively impact overall T cell fitness.
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A notable defect arising from aging is the fading or changing of the original pigments by excessive light.
Fiber misalignment in composites in the form of in-plane waviness and out of-plane wrinkling, is a major defect arising from processes such as hot drape forming.
METHODS The authors conducted a multicenter retrospective case study that included all patients who underwent cranioplasty to correct a skull defect arising from a decompressive craniectomy at 13 centers between 2000 and 2011 and were less than 19 years old at the time of cranioplasty.
Core CdTe and CdSe-based Qdots suffer from low fluorescence quantum yield (QY) and poor fluorescence stability due to surface defect arising from dangling bonds which act as trap sites on their surface.
On the 129 strain, the maternal blood spaces defect arose from isolated loss of Cdkn1c.
The A-T LCLs illustrate a classic model of radiosensitivity and XCIND, whereby increased levels of ROS are secondary to a core DSB recognition and repair defect arising from mutation of a core DDR gene.
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Justyna Jupowicz-Kozak
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