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In-house program Cyndi [38, 39], which uses a multi-objective evolution algorithm method for conformer searching, was chosen by default to generate multiple conformation.

The last option mrpc, which stands for minimum reads per contig, was set to 4 (2 by default) to generate a comprehensive SNP resource only from contigs with at least four reads.

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Similar to [22], all parameters are varied +/−20% from its default value to generate 2000 random parameter sets by using Latin Hypercube Sampling.

For detailed analysis and visualization of results we used PHASE 2.1 software with default parameters to generate estimated haplotypes for each individual in our sample [18], [19].

We used presence-absence of OTU versus dataset matrices generated above as input for the SPADE program [17] and ran the "Presence/Absence Data for Multiple Samples/Quadrats" option using default options to generate OTU richness estimates for our MHB and PAL samples.

They were submitted to WebLogo3 (Crooks et al. 2004) with default options, to generate consensus sequences.

The software package HMMER 2.3.2 [ 28] was used with default settings to generate HMMs.

The default is to generate 5000 intermediate trees from a set of rooted three taxa trees.

A pairwise correlation matrix was then taken as input by APcluster using default parameters to generate the clustering.

It can be used in its default mode to generate HOMA2 results directly comparable with those in the literature.

The Mascot Daemon software (version 2.3.2; Matrix Science, London, UK) was used to perform database searches, using the Thermo Proteome Discoverer v1.3 with default parameters to generate peak lists.

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