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While 12.5 million sequences per sample was sufficient to reach a plateau in the number of transcripts detected, deeper sequence generation resulted in linear increase in average depth of coverage (Fig. 1D).
With deeper sequence coverage, many novel species have been uncovered.
It has been reported that the proportion of polymorphic SNP markers can be increased with deeper sequence coverage.
In a tiered sequencing approach, researchers perform amplicon sequencing on all samples and use their analysis of amplicon data to select critical or representative samples for deeper sequence analysis.
Although targeted single-base assays do require a deeper sequence coverage to adequately select polymorphic SNPs in silico, sequencing costs have dropped drastically so that no major difference in cost between SFP and SNP would happen there.
This DNA-based exclusion list strategy provides an unbiased approach for generating LC-MS/MS data from modified RNAs with deeper sequence coverage than is obtained by standard DDA methods.
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Some wells contain these tests within the shale horizons or the deeper sequences.
For others (5kb_b, 8kb_b), deeper sequencing would be informative.
A deeper sequencing or re-sequencing effort will be required to explore this question thoroughly.
In addition, the sequence of several new MDV-encoded microRNAs were discovered by deeper sequencing.
Deep sequencing revealed 88 variants of potentially biological significance.
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