For the three most represented subtypes in our study, namely, A, D and C, deeper phylogenetic analysis was performed to explore their geographic origin (Fig. 2A, B, C).
A deep process analysis was performed through a LCA approach in order to identify environmental hot-spots in the whole life-cycle.
A cDNA library was prepared and the deep sequencing analysis was performed at the Post Genome Institute (Hongo, Tokyo, Japan).
With the aim of building full MAPK cascades acting in plant reproduction, a deep transcriptomic analysis was performed on pollen and ovule samples in S. chacoense.
For the six samples of test #2, a deep sequencing analysis was performed, with the Ion AmpliSeq™ Cancer Hotspot Panel v2 primer pool and Ion AmpliSeq ™ Master Mix v2.0 (Ion Torrent, Life Technologies, Carlsbad, CA), according to the protocol recommended by the manufacturer.
To evaluate the influence of ethnicity, different kinds of diseases, sample size, and methylation detection method on the relationship between CCNA1 methylation and tumors development, a further deep stratified analysis was performed.
The protein data used in this study were generated in a previous study by Lundberg et al. [ 10], where a deep proteomic analysis was performed on the same three cell lines (A-431, U-2 OS, and U-251MG) used in this study.
In this paper, the structural stratigraphic and paleontological data were used from new studies of outcropping stratigraphic units and from deep wells drilling; Geochemical analysis was performed (Rock Eval pyrolysis, bitumen isotope ratio, etc).
A transcriptomic analysis was performed using deep sequencing (RNA-seq) on five skeletal muscle samples.
In order to get a deep insight into its possible mechanism, phytochemical analysis was performed to reveal the main active components of CDTHD.
