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The deep slope down towards the entrance acted as a loading bay for carts to access the raised floor level directly, when the house was constructed, circa 1800.
Lit only by headlights, it was unnerving to see the car going down a deep slope which appeared to be diving under the trees like a rabbit's burrow.
All remaining 'Deep Slope' samples clustered in two groups corresponding to the haplotypes 'HostD1' and 'HostD2'.
The exception in our study is the 'Deep Slope' habitat that does contain multiple distinct genetic groups (Figures 2, 4).
The remaining 26% of 'Deep Slope' colonies harbored Symbiodinium C3-ff (n = 16) or Symbiodinium C120 (n = 9).
The 'Deep Slope' habitat harbored three additional mtDNA haplotypes: 'HostD1' (n = 62), 'HostD2' (n = 22) and 'HostD3' (n = 3); as well as a few 'HostU' genotypes (n = 9).
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Specific symbiont types were found in association with S. hystrix colonies from either shallow ('Back Reef' and 'Upper Slope') or deep habitats ('Deep Slope').
Corals were cross-transplanted from/to three different habitats: the 'Back Reef' (2 m depth ± 1 m), 'Upper Slope' (6 m depth ± 1 m) and 'Deep Slope' (27 m depth ± 2 m), which are identical to those sampled in Bongaerts et al. [ 27].
and PRIMER 6 (PRIMER-E Ltd .. Across the three sampled habitats, abundances of S. hystrix were significantly lower in the 'Upper Slope' habitat (0.5 ± 0.06 colonies/m) than in the ' Deep Slope' (p < 0.005; Fisher's LSD post-hoc test) and 'Back Reef' (p < 0.05; Fisher's LSD post-hoc test) habitats.
In our previous studies, we identified evidence for anoxia and for water-mass denitrification and sulfate reduction on the relatively deep slope/basin along the continental margin, prior to the extinction (Fig. 1c; Isozaki et al. 2008; Saitoh et al. 2013a, 2013b, 2014a).
Photo-physiological differences were observed between transplants originating from the shallow and deep habitats, with indirect evidence of an increased heterotrophic capacity in native deep-water transplants (from the ' Deep Slope' habitat).
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