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This suggests that the RNA deep sequencing technique is more sensitive in finding differentially expressed genes than the microarray technique.
In this study, we identified the STAT3-mediated miRNA expression profiles through deep sequencing technique, which was followed by validation with qRT-PCR.
Overall, this study provides the first comprehensive analysis of the impact of a parasitoid wasp on its host at the transcriptomic level, using RNA deep sequencing technique.
Four RNA samples containing various tissues and fruit of different development and ripening stages were sequenced using the latest Illumina deep sequencing technique.
To address zinc dependent gene regulation in MAP we performed RNA deep sequencing technique using 50 bp single-ends sequencing on a HiSeq2500 (Illumina, San Diego, CA).
Recent advancements in deep sequencing technique enable the ultra-high-throughput sequencing (called the second- or next- generation sequencing) approaches, to be used for transcriptome, including gene expression, analyses [ 1- 4].
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Parasite DNA from dried blood spots collected from a moderate endemic study area in western Kenya (approximately 15 km by 28 km encompassing more than 80 villages) will be sequenced at a moderately polymorphic gene using deep sequencing techniques.
Using what's known as "deep sequencing" techniques, Kapoor's team fished for nucleic acid sequences (the building blocks of DNA) of known viruses, and found two people who posttransfusion had what looked like a novel flavivirus, the family that includes hepatitis C and human pegivirus.
The number of porcine miRNAs explored has increased in recent times due to the application of new deep sequencing techniques such as those from 454 Life Sciences and Solexa, which are suitable for the sequencing of miRNAs.
The technical limitation in analysis of middle poly(A) sites can be significantly mitigated when genome-tiling microarrays or deep sequencing techniques are employed, which are just beginning to be used in iPS cell research.
However, to provide additional statistical support for our linkage determinations, we developed a Bayesian algorithm incorporating prior probability of linkage and genetic distance data and increased our sensitivity for detecting rare variants in the HIV-1 infected partner that may have been transmitted to the seroconverter using deep sequencing techniques.
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