Sentence examples for deep sequencing on from inspiring English sources
To support this research, we designed an all influenza A-embracing reverse transcription-PCR (RT-PCR) for the generation of DNA from influenza A virus negative strand RNA genome segments for full-length genome deep sequencing on a Genome Sequencer FLX instrument.
The obtained amplicon library was subjected to deep sequencing on the Genome Sequencer Junior instrument (454 Life Sciences).
Zhang et al. applied deep sequencing on serum samples between groups of patients with TB, pneumonia, chronic obstructive pulmonary disease, and lung cancer and healthy controls [25].
Targeted deep sequencing on the injected embryos revealed that 17 out of 17 (100%) or 6 out of 9 (67%) embryos carried targeted point mutations at the target site in the FANCF or DNMT3B gene, respectively (Fig. 1E K).
Moreover, we used deep sequencing on three isolated var clones and were able to assemble them de novo.
To further characterize the metagenomics of 2009 H1N1 infection in humans, we labeled the 17 influenza samples positive for 2009 H1N1 by Virochip with distinct molecular barcodes and analyzed them by paired-end deep sequencing on three lanes of an Illumina Genome Analyzer IIx.
The efficiency of in vivo targeting was assessed by T7E1 assays, site-specific Sanger sequencing, and deep sequencing of on-target and putative off-target sites.
The red star indicated the conversion of C to T. (C) Summary of the deep sequencing of the on-target site for HEK293 site4.
The enriched and barcoded targets were then deep sequenced on an Illumina next generation sequencing platform (Illumina) [ 8].
RNA was extracted from these cells, followed by cDNA synthesis and amplification, and the purified cDNA was deep sequenced on a 454 GS FLX Titanium platform.
Sanger sequencing of the PCR products amplified for mutation validation was carried out by an initial touchdown PCR protocol as described above, using the 192 samples previously deep sequenced on an Illumina platform as DNA template.
