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The notion that the colon's deep crypt pockets provide a protected location that shields stem cells from potentially toxic substances is widely accepted.
In small intestine and colon, in analogy to MIB-1, pThr187-p27 showed positivity in nuclei of deep crypt cells.
In contrast to the observed grn distribution in zebrafish, murine grn is not detected within developing endodermal derivatives, with the exception of adult deep crypt enterocytes [ 42, 46].
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DC, deep crypts; AC, antechamber; D, duct; P, pore; and MF, mucus field.
Such amotile mutants do not successfully colonize the light-organ deep crypts (Graf et al., 1994).
The mucosa including a surface of deep crypts remains intact with supposedly no affection of its function as well as no alteration of tonsillar lymphoid function.
The irregularities in the membrane surface provide deep crypts, which act as binding sites for albumin when the circuit is primed with albumin solution.
Therefore, it remains possible that the defects of the strains in colonizing the light organ are ultimately due, in part, to defects in host recognition/response rather than simply in their failure to migrate into the deep crypts.
In Koropatnick et al., the authors were examining a later time point (24 h post-inoculation), by which time the symbionts have colonized the light organ's deep crypts and undergone many rounds of doubling.
Interestingly, with both the SC and LS antibodies, it appeared that in normal colon tissue there was increased staining in epithelial cells at the mucosal surface with nuclear localization, compared to the deep crypts.
In teratoma cells, PCDGF expression was shown to be essential for tumorigenicity [ 2]. High levels of PCDGF expression are found in rapidly proliferating cells, such as skin cells, deep crypts of gastrointestinal tract, and immune cells.
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