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For all three high-throughput gene expression platforms, differences were considered significant if the false discovery rate-adjusted p-values (q-value) were <0.1, given that the transcripts were deemed expressed.
Affymetrix Human Transcriptome Arrays (HTA 2.0) probed 30,682 annotated transcript clusters, of which 24,371 coding and 5,389 non-coding transcript clusters were deemed expressed in maternal whole blood.
Specifically, of the 1090 Affymetrix probe sets deemed expressed (see Methods) within these 32 ROIs, SAM analyses identified 676 probe sets that showed significant overexpression in metastases (FDR< = 0.05).
Any unigene with at least one successfully mapped sequencing read from either treatment was deemed expressed in that tissue sample.
Hybridisation data were treated following the procedure specific for each platform (see "Methods") to identify genes reproducibly deemed expressed or not expressed.
We examined the proximal promoter regions of three separate gene lists, the top 100 DEX-responsive transcripts generated by GSEA analysis, the 22 091 probe sets deemed expressed in primary chondrocyte cultures and the 1158 transcripts deemed differentially expressed between DEX and vehicle treated cultures by one-Way ANOVA.
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Genes were deemed narrowly expressed if they overlapped at least one transcript alignment in only one of the four tissues and broadly expressed if they overlapped at least one transcript alignment from all four tissues.
Transcript units with a q<0.0001 were deemed differentially expressed.
Gene sets with FDR-corrected p-value lower than 0.05 were deemed differentially expressed.
Hits were deemed differentially expressed relative to control when padjusted < 0.05.
ProbeSets were deemed differentially expressed at p < 0.001 in any given comparison.
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