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The use of positive cases also made it possible to deduce cut-offs of the e-value for each family of proteins to identify homolgs by BLASTP in some taxa where Men proteins were not encoded within men gene clusters.
The graphically deduced cut-off was set at 0.775 0.7500 + 0.025 = 0.775) to include putative reactors falling within this range.
To quantify this size, contrast of each line is analyzed to deduce the cut-off frequency corresponding to 50% of the dynamic range.
In previous 'polyphasic' studies, combining several genotypic and phenotypic methods, a similarity level of ∼80% was deduced as the cut-off level to identify clones among sets of well-defined strains [7], [43].
Cut off.
Their legs cut off.
Cut off the apron.
"Cut off my feet.
Their head cut off.
Molecular weight cut off membrane.
P.J.: "You're cut off".
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