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NCA thus allows the generation of newer hypotheses relating to the conversion of a gene product to an active TF based on how well the gene expression results agree with the deduced activities of their corresponding TFs.
To gain a more complete picture of the expression and deduced activities of components of cytokinin metabolism and signalling in grapevine, the transcript accumulation of the above mentioned 48 cytokinin-related genes was also analysed in a range of other grapevine tissues (Fig. 3).
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By using the connectivity information and gene expression datasets, we used NCA to deduce activities for the NCA-compliant TFs, and numerical values of CS between the TFs and their target genes.
BioQuali uses statistically significant expression profiles to deduce activity levels of transcription factors (TFs), however, it does not assume that non-significant mRNA changes imply a null activity level of the TF.
They deduced TF activities for 154 TFs out of which 127 TFs were found to change their activities significantly.
By using NCA, they deduced the activities of TFs that were affected by the deletion and proposed a mechanism for explaining the growth lag.
The basis for this is deduced structure-activity relationships (SAR), the relationship between physicochemical properties or structural features of a series of molecules and those molecules' biological activities.
Moreover, similar to most other studies, we deduced miRNA activity from target mRNA abundance.
Thus the interaction, if it exists, may be weak, consistent with weak helicase primase interactions in phage T4 and E. coli replication systems that can be deduced by activity assays but elude direct detection methods (Benkovic et al., 2001).
Sriram et al. experimentally verified the NCA-deduced change in TF activity of GR in the glycerol kinase-overexpressing cell line, thus demonstrating the power of NCA for deducing TF activities from gene expression data in a mammalian network.
The company collects the location data and analyzes it to create a map to show where the boat traveled and deduce its activities, such as where it stopped to set out nets or other gear and where and for how long it hauled in a catch.
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CEO of Professional Science Editing for Scientists @ prosciediting.com